The first time you try anything in research it will probably not go perfectly. I call the first run the test run. I plan that the first time I try something I will have to do it again. One to make sure it is reproducable, but also I may be trying to learn how to do the reaction or technique. This gives me a mental freedom if we will see how it goes learn from the first run and adjust. I like to run my science this way. It eliminates frustration of not getting it right the first because I have already plan a learning curve in. I also have more realistically plan a timeline it takes to do something.